Journal: Cell reports

Article Title: RNA-binding protein FXR1 drives cMYC translation by recruiting eIF4F complex to the translation start site

doi: 10.1016/j.celrep.2021.109934

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mutant 3 mRNA 3′UTR target expression clone for Human MYC (see ) , GeneCopoeia , Cat# CS-HmiT102685-MT06-03.

Techniques: Control, Recombinant, Modification, Transfection, Protease Inhibitor, CCK-8 Assay, Bicinchoninic Acid Protein Assay, cDNA Synthesis, SYBR Green Assay, Caspase-Glo Assay, Immunoprecipitation, Plasmid Preparation, Staining, In Situ, Microarray, Western Blot, Expressing, Labeling, Negative Control, Mutagenesis, Software

Journal: Toxicology in vitro : an international journal published in association with BIBRA

Article Title: High-Content Imaging Assays on a Miniaturized 3D Cell Culture Platform

doi: 10.1016/j.tiv.2018.02.014

Figure Lengend Snippet: The Z’ factors and the CV values of the HCI assays performed on the microarray chip platform.

Article Snippet: For robust cell spot attachment and miniaturized 3D cell culture on the microarray chip platform, micropillar chips were coated with 0.01% PMA-OD followed by spotting 60 nL volume of a mixture of 0.003% PLL and 16.66 mM BaCl 2 onto the micropillar chips using a microarray spotter (S+ microarrayer from Samsung Electro-Mechanic Co. or SEMCO).

Techniques: Microarray

Journal: Toxicology in vitro : an international journal published in association with BIBRA

Article Title: High-Content Imaging Assays on a Miniaturized 3D Cell Culture Platform

doi: 10.1016/j.tiv.2018.02.014

Figure Lengend Snippet: Representative images from 72 h-incubated micropillar chips with 3D-cultured Hep3B cells, exposed to menadione (0.7 – 177 μM) for 48 h, and stained with the four fluorescent dyes: (A) TMRM for mitochondrial impairment, (B) Hoechst 33342 for DNA damage, (C) mBCl for changes in intracellular glutathione level, and (D) calcein AM for cell membrane integrity. The dose-dependent effect of menadione for all four assays was clearly observed from the microarray images obtained.

Article Snippet: For robust cell spot attachment and miniaturized 3D cell culture on the microarray chip platform, micropillar chips were coated with 0.01% PMA-OD followed by spotting 60 nL volume of a mixture of 0.003% PLL and 16.66 mM BaCl 2 onto the micropillar chips using a microarray spotter (S+ microarrayer from Samsung Electro-Mechanic Co. or SEMCO).

Techniques: Incubation, Cell Culture, Staining, Membrane, Microarray