Journal: Cell reports

Article Title: RNA-binding protein FXR1 drives cMYC translation by recruiting eIF4F complex to the translation start site

doi: 10.1016/j.celrep.2021.109934

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: 3′UTR target expression clone for Human MYC (see ) , GeneCopoeia , Cat# HmiT102685-MT06.

Techniques: Control, Recombinant, Modification, Transfection, Protease Inhibitor, CCK-8 Assay, Bicinchoninic Acid Protein Assay, cDNA Synthesis, SYBR Green Assay, Caspase-Glo Assay, Immunoprecipitation, Plasmid Preparation, Staining, In Situ, Microarray, Western Blot, Expressing, Labeling, Negative Control, Mutagenesis, Software

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Discovery of RNA Binding Small Molecules Using Small Molecule Microarrays

doi: 10.1007/978-1-4939-6584-7_11

Figure Lengend Snippet: Overview of SMM strategy to identify RNA-binding small molecules. Small molecule libraries prepared in 384-well plates are utilized to print onto isocyanate-functionalized slides by the microarrayer. Prepared SMMs are next incubated with the labeled RNA of interest and scanned to detect hits

Article Snippet: Printing of Small Molecule Microarrays MicroGrid II Microarrayer (BioRobotics) or equivalent.

Techniques: RNA Binding Assay, Incubation, Labeling